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目的探讨血管内皮生长因子(vascular endothelial growth factor,VEGF)-460C/T、-1154G/A、-2578C/A和+936C/T单核苷酸多态性与子宫内膜异位症的关系。方法采用PCR-RFLP方法检测344例子宫内膜异位症患者(病例组)和360名对照妇女(对照组)的VEGF基因多态性位点的基因型频率分布情况。结果VEGF-460C/T和+936C/T多态的基因型和等位基因频率分布在病例组与对照组间差异均无统计学意义(P>0.05)。在病例组和对照组中,VEGF-1154G/A和VEGF-2578C/A多态的基因型及等位基因频率分布差异均有统计学意义(P<0.05);与GA+AA/AA+CA基因型相比,携带GG/CC基因型明显增加内异症的发病风险(OR=1.43,95%CI:1.05～1.96)/(OR=1.47,95%CI:1.09～2.00)。VEGF-460C/T、-1154G/A、-2578C/A多态的单倍型频率在病例组和对照组间差异有统计学意义(P=0.000)。结论1.携带VEGF-1154GG和-2578CC基因型显著增加子宫内膜异位症的发病风险;2.单倍型VEGF-460/-1154/-2578TGC,CAA,TAA和TAC与子宫内膜异位症的发病明显相关。
Objective To investigate the relationship between single nucleotide polymorphisms of vascular endothelial growth factor (VEGF) -460C / T, -1154G / A, -2578C / A and + 936C / T and endometriosis. Methods PCR-RFLP was used to detect the frequency distribution of VEGF gene polymorphism in 344 patients with endometriosis (case group) and 360 control women (control group). Results There was no significant difference in the genotype and allele frequencies of VEGF-460C / T and + 936C / T between the case group and the control group (P> 0.05). The genotype and allele frequencies of VEGF-1154G / A and VEGF-2578C / A polymorphisms were significantly different in case group and control group (P <0.05). Compared with GA + AA / AA + CA (OR = 1.43, 95% CI: 1.05 to 1.96) / (OR = 1.47, 95% CI: 1.09 to 2.00) in the GG / CC genotype. The haplotype frequencies of VEGF-460C / T, -1154G / A and -2578C / A polymorphisms were significantly different between the case group and the control group (P = 0.000). 1. Carrying VEGF-1154GG and -2578CC genotypes significantly increased the risk of endometriosis; 2. The haplotypes VEGF-460 / -1154 / -2578TGC, CAA, TAA and TAC were associated with endometriosis The incidence of disease is clearly related.