论文部分内容阅读
CC83是肿瘤相关糖蛋白TAG-72的第二代单克隆抗体(MAb),它具有比抗TAG MAbs CC49和B72.3更强的亲和常数.临床研究已证实在手持r检测探针方法CC49和B72.3同位素标记的MAbs能有效地检出结肠直肠癌.作者进一步观察同位素标记CC83 MAb的肿瘤定位效果及其安全性.17例复发性结肠直肠癌病人静脉注射~(125)I标记的CC83MAb(2.0mCi~(125)I/0.2mg CC83 MAb),21～28天后进行剖腹探查,常规探查后用手持r-检测探针检测可疑的肿瘤病灶,所用探针1000由放射敏感的碲化镉结晶组成,可以对同位素作出精确的计数.切除常规探查和CC83定位的可疑肿瘤组织,作组化染色.在放免指导手术(RIGS)探查后,重新记录发现结果,并相应修改治疗方案.手术后4周内作甲状腺功能试验、血清癌胚抗原(CEA)、全血计数、凝血参数和肝功能生化测定.记录有关注射同位素标记MAb的并发症和不良反应.结果 在17例注射了CC83MAb,均无并发症或不良反应,其中男9例,女8例,平均年龄60岁(44～77岁),原发肿瘤系结肠癌13例和直肠癌4例.首先怀疑肿瘤复发根据增高CEA值11例、CT扫描4例、结肠镜检和临床判断各1例.传统手术探查疑有肿瘤病灶32处,根据手持r检测探针找出可疑病灶39例,经切除组织的HE染色确诊为肿瘤者27处,该27处均能由同位素标记CC83MAb定位,另有12处为
CC83 is the second-generation monoclonal antibody (MAb) of the tumor-associated glycoprotein TAG-72, which has a stronger affinity constant than the anti-TAG MAbs CC49 and B72.3. Clinical studies have confirmed that in the handheld r-detection probe method CC49 And B72.3 isotope-labeled MAbs can effectively detect colorectal cancer. The authors further observed the tumor localization effect and safety of isotope-labeled CC83 MAb. 17 patients with recurrent colorectal cancer were intravenously labeled with ~(125)I. CC83MAb (2.0mCi 125I/0.2mg CC83 MAb) was used for laparotomy 21 to 28 days later. After routine exploration, a suspected tumor lesion was detected with a hand-held r-detector probe. The probe 1000 used was desensitized by radiation. The crystal composition of cadmium allows accurate counting of isotopes. Resection of suspected tumor tissue by conventional probing and CC83 localization was performed for histochemical staining. After RIGS exploration, the findings were re-recorded and the treatment plan was modified accordingly. The thyroid function test, serum CEA, whole blood counts, coagulation parameters, and biochemical biochemical tests were performed within 4 weeks after the procedure. The complications and adverse reactions related to the injection of isotope-labeled MAbs were recorded. The results were injecting CC83MAb in 17 patients. None Complications or adverse reactions, including 9 males and 8 females, with an average age of 60 years (44-77 years), primary tumors with 13 colon cancers and 4 rectal cancers. The first suspected tumor recurrence was based on increased CEA values in 11 patients. , CT scan in 4 cases, colonoscopy and clinical judgment in 1 case. Traditional surgical exploration suspected 32 tumor lesions, according to the hand-held r detection probe to identify suspicious lesions in 39 cases, the resection of tissue by HE staining confirmed by the tumor 27 At the 27th place, it can be localized by isotope markers CC83MAb, and another 12 are