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目的探讨总抗氧化能力,DNA氧化损伤和单核苷酸多态性(SNP)与结直肠癌的相关风险。方法收集175份人血液样本,其中80例健康对照,67例肠息肉,28例结直肠癌患者。分别采用碱性和经DNA糖基化酶修饰的彗星试验,测定外周血白细胞的直接与DNA氧化损伤,以及血浆中的总抗氧化能力,并采用Applied Biosystems公司的引物系统对单核苷酸多态性进行等位基因鉴别检测。结果结直肠癌患者氧化应激诱导的DNA损伤水平显著高于健康对照组(P<0.05)。肠癌组直接DNA损伤水平也表现出明显升高的趋势(P=0.071)。肠息肉人群总抗氧化能力显著高于对照组。分析肠癌与生活方式(如吸烟和饮酒)关系,肠癌组吸烟率显著高于健康对照组(P<0.01)。分析DNA损伤和修复,代谢和解毒等基因的单核苷酸多态性,hOGG1和GSTM的单核苷酸多态性与结直肠癌之间有一定的相关性。结论高水平的氧化应激诱导的DNA损伤与结直肠癌的发病风险增加相关,是值得进一步研究的潜在的肿瘤生物标志物。
Objective To investigate the total antioxidant capacity, DNA oxidative damage and single nucleotide polymorphisms (SNPs) associated with colorectal cancer risk. Methods 175 human blood samples were collected, including 80 healthy controls, 67 intestinal polyps and 28 patients with colorectal cancer. Alkaline and DNA glycosylase-modified comet assay were used to determine the direct and DNA oxidative damage of peripheral white blood cells and the total anti-oxidative capacity in plasma respectively. Applied Biosystems primer system was used to measure the number of single nucleotide Allele identification of alleles. Results Oxidative stress induced DNA damage in colorectal cancer patients was significantly higher than that in healthy controls (P <0.05). The direct DNA damage in colorectal cancer group also showed a significant increase trend (P = 0.071). Intestinal polyps total antioxidant capacity was significantly higher than the control group. The relationship between colorectal cancer and lifestyle (such as smoking and drinking) was analyzed. The smoking rate in colorectal cancer group was significantly higher than that in healthy control group (P <0.01). Analysis of DNA damage and repair, metabolism and detoxification and other single nucleotide polymorphisms, hOGG1 and GSTM SNP and a certain correlation between colorectal cancer. Conclusions High levels of oxidative stress-induced DNA damage are associated with an increased risk of colorectal cancer and are potential tumor biomarkers for further study.