论文部分内容阅读
目的探讨白介素4(interleukin-4,IL-4)基因的单核苷酸多态性(single nucleotide polymorphisms,SNPs)与皖汉族人群乙型肝炎病毒(hepatitis B virus,HBV)遗传易感性的关系。方法采用病例对照研究,501位慢性乙型肝炎(chronic hepatitis B,CHB)患者和301位急性自限性HBV感染者分别组成病例组和对照组;采用多重单碱基延伸SNP分型技术(Multiplex SNaPshot),检测两组人群的IL-4基因rs2227284G/T、rs2243283C/G和rs2243288A/G 3个标签SNPs位点的单核苷酸多态性,分析两组间的等位基因频率、单体型频率、基因型是否存在统计学差异。结果 IL-4基因3个多态位点的基因型频率在两组间差异均无统计学意义(均有P>0.05);IL-4基因3个态位点最小等位基因及其频率在两组间差异均无统计学意义(均有P>0.05);4个单体型GCA、TCA、TCG及TGG两组间差异均无统计学意义(均有P>0.05)。结论皖汉族人群中IL-4基因的3个tagSNPs(rs2227284,rs2243283和rs2243288)位点的基因多态性与HBV感染的基因易感性可能无相关性。
Objective To investigate the relationship between single nucleotide polymorphisms (SNPs) of interleukin-4 (IL-4) gene and genetic susceptibility to hepatitis B virus (HBV) in Anhui Han population. Methods A case-control study was conducted in 501 patients with chronic hepatitis B (CHB) and 301 patients with acute self-limiting HBV infection. The patients were divided into control group and control group. Multiplexed single base extension SNP (Multiplex SNaPshot). SNPs of rs2227284G / T, rs2243283C / G and rs2243288A / G of IL-4 gene in two groups were detected. The frequencies of alleles, haplotypes Type frequency, genotype whether there is a statistical difference. Results There was no significant difference in genotype frequencies of IL-4 gene polymorphism between the two groups (all P> 0.05). The frequency of IL-4 gene at the three loci was There was no significant difference between the two groups (P> 0.05). There was no significant difference between the four haplotype GCA, TCA, TCG and TGG (P> 0.05). Conclusion The genetic polymorphisms of three tagSNPs (rs2227284, rs2243283 and rs2243288) in IL-4 gene in Anhui Han population may not be associated with the genetic susceptibility to HBV infection.