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目的探讨家蝇蛆粉对小鼠急性毒性和致突变性,以评价其安全性。方法将家蝇3龄幼虫超声破碎的内容物溶液制成干粉,余下的幼虫壳制成壳聚糖,两者按10∶1比例进行组合混匀,制成粉末状家蝇蛆粉。以昆明小鼠为实验对象,采用最大耐受量实验、骨髓微核实验和精子畸形实验,评价家蝇蛆粉对小鼠的急性毒性和致突变性作用。结果小鼠对家蝇蛆粉的最大耐受量为33.0 g/(kg.bw),未见毒性效应或不良反应;家蝇蛆粉2.8,5.5和11.0剂量组雄性小鼠的嗜多染红细胞微核率分别为0.12%,0.14%,0.14%,雌性小鼠的嗜多染红细胞微核率分别为0.14%,0.12%,0.16%,与溶剂对照组比较,差异无统计学意义(P>0.05),小鼠精子畸形率分别为2.3%,1.6%,2.1%,与溶剂对照组比较差异无统计学意义(P>0.05)。结论家蝇蛆粉对小鼠无急性毒性和致突变性。
Objective To investigate the acute toxicity and mutagenicity of housefly maggot powder in mice to evaluate its safety. Methods The third-instar larvae of the housefly were sonicated and the contents of the solution were made into dry powder. The remaining larvae shell was made into chitosan, and the two were mixed in a ratio of 10: 1 to prepare powdery housefly maggot powder. Kunming mice as experimental subjects, the maximum tolerability test, bone marrow micronucleus test and sperm deformity test to evaluate the acute toxicity and mutagenic effects of housefly maggot powder on mice. Results The maximum tolerance of the mice to housefly larvae was 33.0 g / (kg · bw) and no toxic effects or adverse reactions were observed. The numbers of macrophages in the males of 2.8, 5.5 and 11.0 The micronucleus rates were 0.12%, 0.14% and 0.14% respectively. The micronucleus rate of polychromatic erythrocytes in female mice were 0.14%, 0.12% and 0.16%, respectively. There was no significant difference between the two groups (P> 0.05). The rate of sperm deformity in mice was 2.3%, 1.6% and 2.1%, respectively. There was no significant difference between the two groups (P> 0.05). Conclusion Musca domestica powder has no acute toxicity and mutagenicity to mice.