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目的研究柴胡疏肝散对肝郁证大鼠行为学和大鼠前额叶及海马单胺类神经递质的影响。方法 30只大鼠随机均分为空白对照组(对照组)、肝郁证模型组(模型组)、柴胡疏肝散组(治疗组)3组。用慢性束缚应激结合孤养法建立肝郁证大鼠模型,测评开野、蔗糖水实验,采用高效液相色谱法测定大鼠前额叶及海马单胺类神经递质。结果模型组大鼠造模成功,与对照组比较,前额叶皮质及海马中的5-HT、5-HIAA、DA含量均下降,NE含量呈增加趋势,但差异无统计学意义;治疗组与模型组相比,5-HT、5-HIAA与DA含量均增加,差异有统计学意义(P<0.05,P<0.01),NE含量呈减少趋势,但差异无统计学意义(P>0.05)。结论柴胡疏肝散具有治疗肝郁证的作用,其作用机制可能与其能够调节肝郁证大鼠脑5-HT、5-HIAA、NE、DA有关,主要是通过增加海马中的5-HT、5-HIAA、DA水平,从而有效发挥抗肝郁作用。
Objective To investigate the effect of Chaihu Shugan San on the behavior and monoamine neurotransmitters in prefrontal and hippocampus of rats with liver-qi stagnation syndrome. Methods Thirty rats were randomly divided into three groups: control group, control group, model group with liver depression syndrome and model group with Chaihu Shugan Powder (treatment group). Chronic stagnation stress combined with solitary method was used to establish the rat model of liver-qi stagnation. The open-field and sucrose-water experiments were performed. The monoamine neurotransmitters in prefrontal and hippocampus of rats were determined by high performance liquid chromatography. Results Compared with the control group, the content of 5-HT, 5-HIAA and DA in the prefrontal cortex and hippocampus decreased and the content of NE increased, but the difference was not statistically significant Compared with model group, the content of 5-HT, 5-HIAA and DA increased, the difference was statistically significant (P <0.05, P <0.01), NE content decreased, but the difference was not statistically significant (P> 0.05) . Conclusion Chaihu Shugan San has the effect of treating stagnation of liver-qi. Its mechanism may be related to its regulation of 5-HT, 5-HIAA, NE, DA in rats with liver-qi stagnation syndrome, mainly by increasing 5-HT , 5-HIAA, DA levels, which effectively play a role in anti-liver depression.