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设计合成了新的(2-(2’-吡啶)苯并咪唑)(L-丙氨酸根)铜(II)配合物:[Cu(HPB)(L-Ala)(ClO4)(H2O)]2 H2O[HPB=2-(2’-吡啶)苯并咪唑,L-Ala=L-丙氨酸根].应用元素分析、红外光谱、紫外可见光谱、摩尔电导率、电喷雾质谱及X射线单晶衍射等方法对配合物的组成及结构进行了表征.该配合物晶体属单斜晶系,P21空间群,晶胞参数:a=1.1900(2)nm,b=0.80500(16)nm,c=1.9700(4)nm,β=94.78(3)°,Z=2,Dc=1.672 g cm-3,F(000)=968,残差因子R1=0.0427,wR2=0.1106[I>2σ(I)],S=0.999.在配合物分子中,2-(2’-吡啶)苯并咪唑和L-丙氨酸根以双齿配位方式在分子平面上与中心铜(II)离子配位,而水分子及高氯酸根单齿弱配位于分子轴向上,构成了一拉长的八面体结构.利用二倍试管稀释法测定了配合物的抗菌活性,并且研究了配合物对pBR 322 DNA的断裂作用.结果表明,该配合物对枯草杆菌(B.subtilis,G+),金黄色葡萄球菌(S.aureus,G+),大肠杆菌(E.coil,G-)和沙门氏杆菌(Salmonella,G-)具有良好的抑制活性,最小抑菌浓度为50～80μg mL-1,在维生素C存在下能够通过羟基自由基OH氧化断裂pBR 322 DNA双螺旋结构.
(Cu (HPB) (L-Ala) (ClO4) (H2O)] 2 has been designed and synthesized with the new copper (II) H2O [HPB = 2- (2’-pyridine) benzimidazole, L-Ala = L-alanine] The crystal structure of the complex is monoclinic, P21 space group with unit cell parameters: a = 1.1900 (2) nm, b = 0.80500 (16) nm, c = 1.9700 (4) nm, β = 94.78 (3) °, Z = 2, Dc = 1.672 g cm-3 and F (000) = 968. The residual factor R1 = 0.0427 and wR2 = 0.1106 [I> ], S = 0.999. In complex molecules, 2- (2’-pyridyl) benzimidazole and L-alanine coordinate with the central copper (II) ion in a bidentate manner on the molecular plane, while The water molecules and perchlorate groups were weakly coordinated to the molecular axis, forming an elongated octahedral structure.The antibacterial activity of the complex was determined by double tube dilution method and the effect of the complex on pBR 322 DNA The results show that the complex has strong antibacterial activity against B. subtilis (G +), S. aureus (G +), E. coli (G-) and sand Bacillus (Salmonella, G-) having excellent inhibitory activity, the minimum inhibitory concentration of 50 ~ 80μg mL-1, can be 322 DNA double helix by oxidative cleavage pBR hydroxyl radical OH in the presence of vitamin C.