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目的:探讨核转录因子kB(NF-kB)在牙龈成纤维细胞(GF)中活性的变化及其在牙周病发生中的意义,探讨NF-kB在牙周病调控中的作用及途径。方法:采用免疫细胞化学染色法,观察NF-kB在GF中活性的变化。结果:以10μg/mL的LPS作为有效刺激浓度刺激GF,6h后NF-kB核转位达峰值;以10～100μg/mL浓度的LPS作用GF时,NF-KB激活程度不同,表明10～100μg/mL浓度的LPS是NF-kB激活的有效浓度。结论:健康牙龈组织GF细胞中NF-KB处于非激活状态,LPS可诱导人GF细胞中NF-kB激活发生核转位,LPS通过调控NF-KB的激活调节炎症因子对牙周组织的破坏。
Objective: To investigate the changes of NF-κB activity in gingival fibroblasts (GF) and its significance in the pathogenesis of periodontal disease and to explore the role of NF-κB in the regulation of periodontal disease. Methods: Immunocytochemical staining was used to observe the changes of NF-kB in GF. RESULTS: GF was stimulated with 10μg / mL LPS for 6 hours and NF-κB nuclear translocation peaked at 6 hours. The activation of NF-κB was different between the concentrations of 10 ~ 100μg / mL LPS, indicating that 10 ~ 100μg LPS at a concentration of / mL is an effective concentration of NF-kB activation. CONCLUSION: NF-KB in GF cells of healthy gingival tissues is inactive. LPS can induce nuclear translocation of NF-κB activation in human GF cells. LPS regulates the destruction of periodontal tissues by regulating the activation of NF-κB.