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目的:探讨人乳头瘤病毒（human papillomavirus，HPV）16/18E6蛋白在肺腺癌患者组织和癌旁组织中的表达情况及临床意义。方法:选取60例经病理确诊为肺腺癌的患者的病理组织标本及对应的癌旁组织，免疫组化检测HPV16/18E6在肺腺癌患者组织中的表达情况，n χ2检验分析HPV16/18E6的表达差异与肺腺癌患者临床特征的关系。利用细胞转染技术建立A549肺腺癌HPV16/18 E6高表达细胞株，通过噻唑蓝法比较E6组、对照组和A549组对顺铂耐药性的差异，并计算半抑制浓度（IC50）。n 结果:60例入选病例中，男性29例（48%），女性31例（52%），年龄（55.5±2.4）岁。肺腺癌患者病理组织中HPV16/18E6阳性率为48%（29/60），高于癌旁组织18%（11/60），差异有统计学意义（n P<0.05）；不同TNM分期、年龄、性别、吸烟及肿瘤大小的患者中，HPV16/18E6阳性率差异均无统计学意义。E6组顺铂IC50（111 mg/L）高于对照组（75 mg/L）和A549组（79 mg/L），差异有统计学意义（n P<0.05）。n 结论:高危型HPV16/18E6在肺腺癌患者中高表达，且与TNM分期、年龄、性别、吸烟及肿瘤大小无关。高危型HPV16/18E6对化疗药物的耐药性有影响。“,”Objective:To analyze the expression and clinical significance of human papillomavirus (HPV) 16/18E6 in lung adenocarcinoma tissues and adjacent specimens.Methods:Sixty patients with pathologically confirmed lung adenocarcinoma treated with surgical resection were included in the study. To determine the expression of HPV16/18 E6, carcinoma specimens and adjacent tissues were collected and detected using immunohistochemistry. Then χn 2 test was used to analyze the relationship between HPV16/18 E6 expression and the clinical features of lung adenocarcinoma. Using the cell transfection technique, the A549 lung adenocarcinoma cell line with high HPV16/18E6 expression was established, and the differences in cisplatin resistance between the E6, control (Ctl), and A549 groups were compared using the MTT assay.n Results:Of the 60 cases, 29 (48%) were male and 31 (52%) were female, with an average age of (55.5±2.4) years. In lung adenocarcinoma specimens, the positive expression rate of HPV16/18 E6 was 48% (29/60), which was higher than that observed in adjacent tissues (18%; 11/60), demonstrating a statistically significant difference n (P<0.05n );n the differences between the positive expression rate of HPV16/18E6 and TNM stage, age, gender, smoking, and tumor size were not statistically significant. In the E6 A549 lung adenocarcinoma group, cisplatin resistance was higher than that in the Ctl and A549 groups, demonstrating a statistically significant differencen (P<0.05n ). Finally, the half-maximal inhibitory concentration (ICn 50) of cisplatin in E6 (111 mg/L)was higher than that in Ctl(75 mg/L) and A549(79 mg/L) groups, demonstrating a statistically significant difference n (P<0.05n ).Conclusions:High-risk HPV16/18E6 has a higher positive expression rate in lung adenocarcinoma, independent of TNM stage, age, gender, smoking, and tumor size, and may influence resistance to chemotherapy drugs.