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目的:通过定量检测上皮性卵巢癌患者血清DNA水平,探索可用于上皮性卵巢癌诊断的新方法。方法:用微量基因组DNA抽提试剂盒提取31例上皮性卵巢癌、10例交界性上皮性卵巢肿瘤及13例卵巢良性病变患者血清DNA,用SYBRGreen I荧光染色法测定其含量。结果:上皮性卵巢癌患者血清DNA含量(57.48±76.21 ng/ml)高于交界性上皮性卵巢肿瘤(24.90±20.66 ng/ml)和卵巢良性病变患者(20.13±10.98 ng/ml),差异有统计学意义(t=-2.378,P<0.05)。而交界性上皮性卵巢肿瘤和卵巢良性病变患者血清DNA水平则无明显差异(t=-0.544,P>0.05)。结论:上皮性卵巢癌患者血清DNA定量检测有可能成为辅助上皮性卵巢癌诊断的新方法。
OBJECTIVE: To explore a new method for the diagnosis of epithelial ovarian cancer by quantitative detection of serum DNA levels in patients with epithelial ovarian cancer. Methods: Serum DNA of 31 patients with epithelial ovarian cancer, 10 patients with borderline epithelial ovarian tumor and 13 patients with benign ovarian disease was extracted by using the kit of DNA extraction kit. The content of serum was determined by SYBR Green I fluorescence staining. Results: The serum DNA level in patients with epithelial ovarian cancer (57.48 ± 76.21 ng / ml) was higher than that in borderline epithelial ovarian tumors (24.90 ± 20.66 ng / ml) and benign ovarian lesions (20.13 ± 10.98 ng / ml) Statistical significance (t = -2.378, P <0.05). There was no significant difference in serum DNA levels between borderline epithelial ovarian tumors and benign ovarian lesions (t = -0.544, P> 0.05). Conclusion: Quantitative detection of serum DNA in patients with epithelial ovarian cancer may become a new method for the diagnosis of epithelial ovarian cancer.