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目的:建立HPLC测定关节解痛膏中辣椒素、二氢辣椒素、欧前胡素和异欧前胡素含量的方法。方法:CAPCELL PAK C18色谱柱(250 mm×4.6 mm,5μm);流速:0.8 ml·min-1;流动相A:甲醇-乙腈(1∶1),流动相B:1%磷酸溶液,梯度洗脱;检测波长分别为280 nm(辣椒素和二氢辣椒素)和254 nm(欧前胡素和异欧前胡素)。结果:辣椒素、二氢辣椒素、欧前胡素和异欧前胡素分别在0.298~5.956μg(r=0.999 8)、0.152~3.044μg(r=0.999 6)、0.018~0.352μg(r=0.999 5)、0.010~0.204μg(r=0.999 3)范围内进样量与峰面积呈良好的线性关系,平均加样回收率分别为97.8%、97.0%、96.6%、98.1%,RSD分别为1.02%、0.76%、0.65%、1.35%(n=6)。结论:方法简便、准确、灵敏、重复性好,可作为关节解痛膏中辣椒素、二氢辣椒素、欧前胡素和异欧前胡素的含量控制方法。
OBJECTIVE: To establish a HPLC method for the determination of capsaicin, capsaicin, imperatorin and isoimperatorin in Jiejie Tongxiang cream. Method: CAPCELL PAK C18 column (250 mm × 4.6 mm, 5 μm); flow rate: 0.8 ml · min -1; mobile phase A: methanol-acetonitrile Off; the detection wavelength was 280 nm (capsaicin and dihydro capsaicin) and 254 nm (imperatorin and isoimperatorin). Results: The plasma concentrations of capsaicin, capsaicin, imperatorin and isoimperatorin range from 0.298 to 5.956 μg (r = 0.999 8), from 0.152 to 3.044 μg (r = 0.999 6), from 0.018 to 0.352 μg = 0.999 5) and 0.010 ~ 0.204μg (r = 0.999 3), respectively. The average recoveries were 97.8%, 97.0%, 96.6% and 98.1%, respectively Was 1.02%, 0.76%, 0.65%, 1.35% (n = 6). Conclusion: The method is simple, accurate, sensitive and reproducible. It can be used as a method for the content control of capsaicin, capsaicin, imperatorin and isoimperatorin in Jietong Tongjie.