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目的探讨三氧化二砷(As2O3)联合放射治疗对人鼻咽癌细胞系(CNE-1)的生长抑制作用。方法采用四甲基噻唑蓝(MTT)比色法和流式细胞仪(FCM)碘化丙啶(PI)染色法观察不同浓度As2O3与放射联合和单纯As2O3对CNE-1的杀伤和诱导凋亡作用。结果细胞生长抑制随着As2O3剂量的增加而增强;放射组的细胞存活率均低于单纯As2O3组。结论As2O3对鼻咽癌细胞株CNE-1有明显的放射增敏效应。
Objective To investigate the inhibitory effect of arsenic trioxide (As2O3) combined with radiotherapy on the growth of human nasopharyngeal carcinoma cell line (CNE-1). Methods MTT assay and flow cytometry (FCM) propidium iodide (PI) staining were used to observe the killing and apoptosis of CNE-1 induced by different concentrations of As2O3 in combination with radiation and As2O3 alone effect. Results The cell growth inhibition increased with the increase of dose of As2O3. The cell viability of the irradiated group was lower than that of the pure As2O3 group. Conclusion As2O3 has obvious radiosensitizing effect on nasopharyngeal carcinoma cell line CNE-1.