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采用高效液相色谱法分析测定番茄及其种植土壤中的霜脲氰残留,对样品的提取净化条件进行了优化。结果表明:以50 mL二氯甲烷为提取溶剂,番茄样品高速匀质2 min,土壤样品恒温(30℃)振荡提取60 min时,霜脲氰的提取净化效果较好。在试验所设色谱条件下,霜脲氰标准溶液的相对保留时间为7.43 min;在0.4~80 ng范围内,霜脲氰峰面积与进样量线性关系良好。添加回收试验结果表明:该方法的最低检出限为0.04 mg/kg,在灵敏度、准确度及精密度上均符合农药残留分析的要求,可用于实际生产中番茄和土壤样品中霜脲氰的残留检测。
High performance liquid chromatography (HPLC) was used to determine the residue of cymoxanil in tomato and its planting soil, and the extraction and purification conditions of the sample were optimized. The results showed that when 50 mL dichloromethane was used as the extraction solvent, the tomato samples were homogenized at high speed for 2 min and the soil samples were extracted by shaking at 30 ℃ for 60 min. Under the chromatographic conditions, the relative retention time of cream urea standard solution was 7.43 min. In the range of 0.4 ~ 80 ng, the area of the urea peak had a good linear relationship with the injection volume. The recovery test results show that the method has the lowest detection limit of 0.04 mg / kg, which meets the requirements of pesticide residue analysis in terms of sensitivity, accuracy and precision, and can be used in the actual production of cymoxanil in tomato and soil samples Residue detection.