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发根农杆菌（Ａｇｒｏｂａｃｔｅｒｉｕｍｒｈｉｚｏｇｅｎｅｓ）Ａ４（ＡＴＣＣ３１７８９）感染短叶红豆杉（Ｔａｘｕｓｂｒｅｖｉｆｏｌｉ－ａ）芽外植体，３０～３５ｄ后可诱导出毛状根，４０ｄ后转化率可达３０％，毛状根经农杆碱单克隆抗体酶联免疫分析，证明已被转化。毛状根生长速度较快，５株毛状根在无激素的Ｂ５液体培养基中悬浮培养２０ｄ，生物量平均增加约９倍，是同等条件下短叶红豆杉愈伤组织液体悬浮培养的２．９倍。经紫杉醇单克隆抗体酶联免疫反应检测，毛状根中含有紫杉醇，其含量为短叶红豆杉愈伤组织的１．３～８．０倍，悬浮培养过程中，毛状根也向培养液中分泌０．０１～０．０３ｍｇ／Ｌ的紫杉醇。
Hairy roots were induced after 30-35 days in Agrobacterium rhizogenes A4 (ATCC31789) infected shoots of Taxus brevifoli-a, and the rate of transformation reached 30% after 40 days. Agrobacterium monoclonal antibody enzyme-linked immunosorbent assay proved to have been transformed. Hairy roots grew faster, and 5 hairy roots were cultured in hormone-free B5 liquid medium for 20 days. The biomass increased by about 9 times on average, which was the result of suspension culture of Callus of Taxus chinensis under the same conditions .9 times. Paclitaxel monoclonal antibody enzyme-linked immunosorbent assay, hairy roots containing paclitaxel, the content of the callus of the Brassica embryos 1.3 to 8.0 times the suspension culture, hairy roots also to the culture medium Secretion of 0.01 ~ 0.03mg / L of paclitaxel.