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离体技术(in vitro technology)一词最近才出现在植物生物工程的文献中,它包含离体组织及细胞培养技术;还包括细胞杂交、突变体筛选等离体遗传操作技术。植物离体技术已逐步应用于植物育种实践,在应用上有广阔前景的几个领域如下。利用幼胚培养技术获得远缘杂种幼胚培养并非新技术,早在1925年莱巴赫就用离体培养幼胚的方法培养出在自然条件下不能存活的亚麻属的种间杂种。现在已经知
The term in vitro technology has recently appeared in the literature on plant bioengineering, which includes ex vivo tissue and cell culture techniques, as well as cell-based hybridization and mutant screening of genetic manipulation techniques. Plant in vitro technology has been gradually applied to plant breeding practice, there are several areas of promising applications are as follows. The use of immature embryo culture technology to obtain distant hybrid immature embryo culture is not a new technology, as early as 1925, Leibach on the in vitro culture of immature embryos cultured in nature can not survive in flax interspecific hybrids. Now known