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目的探讨中药骨康含药血清培养脂肪干细胞移植促进骨折愈合过程中骨折端骨痂的生长情况。方法制备中药骨康含药血清并进行脂肪干细胞培养,移植脂肪干细胞到骨折部位,用X线观察骨折部位骨痂生长情况。结果第3代脂肪干细胞流式鉴定CD90阳性,CD34、CD49低表达。第1、2周各组骨折愈合情况差异无统计学意义。第4周干预组的骨折已完全愈合,断端边缘消失,骨折线局部密度增高,断端的塑形改造较其他各组明显提前;对照组骨折有较多骨痂形成,但断端边缘仍可见,骨折线局部密度稍淡;空白组骨折线模糊,可见骨痂形成,断端边缘清晰可见,整体愈合情况不如干预组和对照组。干预组、对照组DR片评分与空白组比较差异有统计学意义(P<0.05),干预组与对照组差异亦有统计学意义(P<0.05)。结论中药骨康含药血清培养脂肪干细胞移植可促进骨质疏松性骨折愈合。
Objective To investigate the growth of callus in the fracture site during the process of fracture healing by transplanting adipose-derived stem cells with traditional Chinese medicine GuKang drug-containing serum. Methods Preparation of traditional Chinese medicine GuKang drug serum and adipose-derived stem cell culture, transplantation of adipose stem cells to the fracture site, X-ray observation of fracture site callus growth. Results The third generation of ADSCs were identified by flow cytometry. The expression of CD90, CD34 and CD49 were low. There was no significant difference between the two groups in the first and second week of fracture healing. At the 4th week, the fracture in the intervention group was completely healed, the edge of the broken edge disappeared, the local density of the fracture line increased, and the plastic deformation of the broken end was obviously advanced compared with the other groups. In the control group, more callus was formed in the fracture, Visible, the local density of the fracture line slightly lighter; blank group of fracture line fuzzy, callus formation can be seen, the edge of the broken end is clearly visible, the overall healing situation than the intervention group and the control group. There was significant difference between the intervention group and the control group (P <0.05), the difference between the intervention group and the control group was also statistically significant (P <0.05). Conclusion Traditional Chinese medicine GuKang serum containing adipose-derived stem cells can promote osteoporotic fracture healing.