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作为一种交替的和补充的选育玉米自交系的方法,花药培养产生单倍体世代已引起许多研究者的兴趣,并已取得很大进展(Kuo 等,1986)。愈伤组织诱导率对育种程序中的单倍体世代法的实际应用是一个主要障碍。然而,愈伤组织诱导率在过去的十年中已有较大的提高。中国科学院植物细胞和组织培养研究室的研究人员(1975)把蔗糖浓度从6%增加到12~
As an alternative and complementary method to breed maize inbred lines, anther culture to generate haploid generations has attracted the interest of many researchers and great progress has been made (Kuo et al., 1986). The callus induction rate is a major obstacle to the practical application of haploid generation in breeding programs. However, the callus induction rate has been greatly increased in the past decade. Researchers at the Plant Cell and Tissue Culture Laboratory of the Chinese Academy of Sciences (1975) increased the sucrose concentration from 6% to 12 ~