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本文研究了麻疹病毒在体外胸腺上皮细胞(TE细胞)培养中的复制.16只胎、婴儿胸腺用常规法粉碎成0.3～0.4mm大小,移于含促生长激素(HDM)和新生儿脐带血清的培养液中.新生细胞吸附平皿后,改用无血清HDM培养5～10天接种麻疹病毒,第1～15天用免疫荧光法(IFA)检查.TE细胞培养液接种Vero细胞.用TE-4(抗皮质、髓质)、TE-15、TE-19(抗胸腺小体)、A2B5C抗CQ神经节苷脂)、HFI(抗角蛋白)单克隆抗体(McAb)检查感染麻疹病毒的TE细胞表型特征.
In this paper, the replication of the measles virus in vitro was studied in the culture of thymus epithelial cells (TE cells) .Three females, thymus of the infants were crushed to the size of 0.3-0.4 mm by conventional method, and then were transplanted in the culture medium containing growth hormone (HDM) and neonatal umbilical cord serum Of the culture medium.Newcell cells after adsorption plate, instead of using serum-free HDM cultured 5 to 10 days of measles virus inoculation, 1 to 15 days by immunofluorescence (IFA) examination.TE cell culture medium was inoculated Vero cells.Using TE- 4 (anti-cortex, medulla), TE-15, TE-19 (anti-thymus), A2B5C anti-CQ ganglioside), HFI (anti-keratin) monoclonal antibody Cell phenotypic characteristics.