论文部分内容阅读
由于蛋白质分子本身的大分子体积和易变性空间结构,导致蛋白质印迹研究目前仍然面临重大挑战。本文提出一种制备磁性蛋白质表面印迹新方法。首先以溶剂热法一步合成羧基修饰的磁性内核,然后一步实现表面双键修饰,最后以改进的丙烯酰胺印迹体系实现印迹包裹。这种制备方法避免了多步修饰带来的纳米颗粒交联和包裹层过厚等问题。高的饱和磁感应强度表明磁性纳米颗粒具有非常快速的磁响应。吸附实验结果表明I表面印迹聚合物能在10min达到吸附平衡,具有非常快的吸附动力学性能,且符合准二级吸附动力学模型。进一步吸附选择性实验结果显示印迹聚合物具有选择性吸附目标蛋白。
Due to the macromolecular volume and the variability of the spatial structure of the protein molecule itself, there are still major challenges in the study of western blotting. This paper presents a new method for preparing magnetic protein surface imprinting. Firstly, the carboxyl-modified magnetic core was synthesized by one-step solvothermal method, then the double bonds of the surface were modified in one step. Finally, the imprinting was achieved by the improved acrylamide imprinting system. The preparation method avoids the problems of multi-step modification such as crosslinking of the nano-particles and excessive thickness of the coating layer. High saturation magnetization indicates that the magnetic nanoparticles have a very fast magnetic response. The results of adsorption experiments show that I surface imprinted polymer can reach adsorption equilibrium in 10 min, has very fast adsorption kinetics and complies with quasi-second-order adsorption kinetics model. Further adsorption selectivity experiment results show that the imprinted polymer has a selective adsorption target protein.