卵巢癌患者外周血单个核细胞SULT1A1、ICAM5基因单核苷酸多态性的研究

• 作 者: 张鹏 刘爱云 王洋
• 机 构: 山东大学齐鲁医院桓台分院(桓台县人民医院),
• 出 处: 社区医学杂志
• 发表日期: 2011年16期
• 关键词: 卵巢 ICAM5 SULT1A1 硫酸基转移酶 细胞间粘附分子 基因型分布 女性卵巢 单核苷酸多态性 癌易感性 等位基因频率

目的探讨硫酸基转移酶(SULT)1A1、细胞间粘附分子(ICAM5)基因多态性与女性卵巢癌易感性的关系。方法采外周血DNA后用等位基因特异性扩增法(ASA)检测淄博市92例正常对照者和97例卵巢癌患者SULT1A1、ICAM5基因多态性分布,分别比较正常组和病例组各种基因型分布频率的差异,并进行统计学分析。结果 (1)SULT1A1 Arg/Arg、Arg/His、His/His三种基因型分布在对照组和病例组之间的差异无显著意义(P=0.082);但与Arg/Arg基因型相比,Arg/His、His/His危险度呈增加趋势(P=0.035);病例组和对照组His等位基因频率差异有统计学意义.(2)ICAM5基因各基因型分布频率在病例组和对照组间的差异无显著意义(P=0.927)。结论 SULT1A1 His等位基因与汉族女性卵巢癌的发生相关,可能成为卵巢癌早期基因诊断的一个指标。

Objective To investigate the relationship between SULT1A and ICAM5 gene polymorphisms and susceptibility to ovarian cancer in women. Methods The allele-specific amplification (ASA) method was used to detect the polymorphisms of SULT1A1 and ICAM5 in 92 cases of normal controls and 97 cases of ovarian cancer patients in Zibo City, Genotype distribution frequency differences, and statistical analysis. Results (1) There was no significant difference in the distribution of SULT1A1 Arg / Arg, Arg / His, His / His genotype between the control group and the case group (P = 0.082) The risk of Arg / His and His / His showed an increasing trend (P = 0.035). There was significant difference in the frequencies of His alleles between the case group and the control group. (2) The distribution frequency of ICAM5 gene in case group and control group There was no significant difference between the two groups (P = 0.927). Conclusion The SULT1A1 His allele is associated with the occurrence of ovarian cancer in Han women and may be an indicator of early gene diagnosis of ovarian cancer.