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目的 :观察霉酚酸酯 (MMF)对炎症因子刺激下人脐静脉内皮细胞白细胞介素 6 (IL 6 )分泌的影响。 方法 :以TNFα(10 μg/L)刺激人脐静脉内皮细胞 ,同时加用霉酚酸 (MPA ,10或 5 0 μmol/L)和鸟嘌呤核苷 (10 0μmol/L)共同孵育 ,采用ELISA法检测细胞上清IL 6的分泌量。 结果 :TNFα刺激内皮细胞 2 4h后 ,内皮细胞分泌的IL 6明显增加 [(114 85± 5 2 1 3)vs (12 91 7± 10 8 6 )ng/ (L·10 6cell) ,P <0 0 1]。MPA可以抑制TNFα诱导的内皮细胞IL 6的分泌 [(485 7 7± 2 34 5 )vs (12 818 7± 72 5 6 )ng/ (L·10 6cell) ,P <0 0 1],且随着MPA剂量的增加 ,抑制作用加强 ;添加外源性的鸟嘌呤核苷不能逆转MPA抑制内皮细胞分泌IL 6的作用 [(42 5 4 0± 6 72 8)vs (374 7 3±82 1 8)ng/ (L·10 6Cell],P >0 0 5 )。 结论 :MMF可以抑制炎症因子诱导的内皮细胞IL 6的分泌 ,且此作用与抑制嘌呤代谢无关。MMF的这一作用可能是其对血管病变和炎症反应具有良好疗效的机制之一
Objective: To observe the effect of mycophenolate mofetil (MMF) on the secretion of interleukin 6 (IL 6) in human umbilical vein endothelial cells stimulated by inflammatory cytokines. Methods: Human umbilical vein endothelial cells were stimulated with TNFα (10 μg / L) and incubated with mycophenolic acid (MPA, 10 or 50 μmol / L) and guanosine nucleoside (100 μmol / L) Method to detect the secretion of IL 6 in the cell supernatant. Results: After IL-4 stimulated endothelial cells for 24 hours, the IL 6 secreted by endothelial cells increased significantly (114 85 ± 5 2 1 3) vs (12 91 ± 10 8 6) ng / (L · 10 6 cells), P <0 0 1]. MPA could inhibit TNFα-induced IL 6 secretion in endothelial cells [(485 7 ± 2 34 5) vs (12 818 7 ± 72 56) ng / (L · 10 6 cells), P <0.01) The increase of MPA dosage and the inhibition were strengthened. The addition of exogenous guanosine did not reverse the effect of MPA on the secretion of IL-6 by endothelial cells [(42 54 0 ± 6 72 8) vs (374 7 ± 82 18 ) ng / (L · 10 6 Cell], P> 0 0 5). CONCLUSION: MMF can inhibit IL-6 secretion induced by inflammatory cytokines and this effect is not related to the inhibition of purine metabolism. This effect of MMF may be one of the mechanisms that has a good effect on vascular lesions and inflammatory responses