Therapeutic effect of nerve growth factor on cerebral infarction in dogs using the hemisphere anomal

• 作 者: Yong Wang Hui Zhang Zhe Wang Zuojun Geng Huaijun Liu Haiqing Yang Peng Song Qing Liu
• 机 构: Department of Radiology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei
• 出 处: 中国神经再生研究（英文版）
• 发表日期: 2012年24期
• 关键词: diffusion-weighted MRI nerve growth factor hemisphere anomalous volume ratio cer

A model of focal cerebral ischemic infarction was established in dogs through middle cerebral artery occlusion of the right side. Thirty minutes after occlusion, models were injected with nerve growth factor adjacent to the infarct locus. The therapeutic effect of nerve growth factor against cerebral infarction was assessed using the hemisphere anomalous volume ratio, a quantitative index of diffusion-weighted MRI. At 6 hours, 24 hours, 7 days and 3 months after modeling, the hemisphere anomalous volume ratio was significantly reduced after treatment with nerve growth factor. Hematoxylin-eosin staining, immunohistochemistry, electron microscopy and neurological function scores showed that infarct defects were slightly reduced and neurological function significantly improved after nerve growth factor treatment. This result was consistent with diffusion-weighted MRI measurements. Experimental findings indicate that nerve growth factor can protect against cerebral infarction, and that the hemisphere anomalous volume ratio of diffusion-weighted MRI can be used to evaluate the therapeutic effect.