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利用免疫荧光技术和激光扫描共聚焦显微镜（共焦镜）技术检测了地塞米松介导小鼠腹腔巨噬细胞凋亡时凋亡抑制基因 Ｂｃｌ－２表达的时空变化。结果显示，免疫荧光技术和共焦镜技术可对细胞内的蛋白表达作定量和定位检测，凋亡巨噬细胞内 Ｂｃｌ－２ 逐渐减少；核中 Ｂｃｌ－２相对量逐渐减少，胞浆中 Ｂｃｌ－２相对量逐渐增多；Ｂｃｌ－２表达和地塞米松介导的巨噬细胞凋亡是极显著负相关。结果表明，地塞米松介导巨噬细胞凋亡时，凋亡抑制基因Ｂｃｌ－２表达发生时空改变，Ｂｃｌ－２表达极显著抑制地塞米松介导巨噬细胞凋亡。免疫荧光技术结合共焦镜技术是一种理想的蛋白表达检测方法。
Immunofluorescence and laser scanning confocal microscopy (confocal microscopy) were used to detect the temporal and spatial changes of Bcl-2 expression induced by dexamethasone in mouse peritoneal macrophages. The results showed that the expression of Bcl-2 in apoptotic macrophages was decreased by immunofluorescence and confocal microscopy. The relative amount of Bcl-2 in the nucleus decreased gradually and the expression of Bcl-2 in cytoplasm -2 gradually increased; Bcl-2 expression and dexamethasone-mediated apoptosis of macrophages is a very significant negative correlation. The results showed that when the dexamethasone mediated macrophage apoptosis, the expression of Bcl-2 had a spatiotemporal change, and Bcl-2 expression significantly inhibited dexamethasone-mediated macrophage apoptosis. Immunofluorescence combined with confocal microscopy is an ideal method for detecting protein expression.