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在研究纳米材料的生物效应时,首先需要对纳米材料在生物介质中的物理化学性质进行全面详细的表征。受生物介质中高蛋白浓度和高离子强度的影响,纳米颗粒在生物介质中极易团聚,对纳米颗粒在生物分散体系中的粒度表征十分困难。本实验采用同步辐射X射线小角散射法(SR-SAXS)研究纳米Fe、Fe2O3和Fe3O4颗粒及其在磷酸盐缓冲液(PBS)、DMEM培养基分散体系中的粒度分布,并与TEM结果进行比较。结果显示SR-SAXS可准确测试纳米Fe、Fe2O3和Fe3O4颗粒的粒度分布,且不受分散体系和分散浓度的影响。表明SR-SAXS可很好地应用于测试稳定性较差的非均匀分散体系中纳米颗粒的粒度分析。
In studying the biological effects of nanomaterials, it is first necessary to conduct a comprehensive and detailed characterization of the physicochemical properties of nanomaterials in biological media. Under the influence of high protein concentration and high ionic strength in biological medium, nanoparticles are easily aggregated in biological media, and it is very difficult to characterize the particle size of nanoparticles in the biological dispersion system. In this study, the particle size distribution of nano-Fe, Fe2O3 and Fe3O4 particles and their dispersion in phosphate buffered saline (PBS) and DMEM medium were studied by synchrotron radiation X-ray small angle scattering (SR-SAXS) . The results show that SR-SAXS can accurately test the particle size distribution of Fe, Fe2O3 and Fe3O4 nano-particles, and is not affected by the dispersion system and dispersion concentration. It shows that SR-SAXS can be applied well to the particle size analysis of nanoparticles in non-uniform dispersion with poor stability test.