肝再生时前列腺素E_2调控枯否细胞的白介素6生成

• 作 者: 张延龄
• 出 处: 国外医学.创伤与外科基本问题分册
• 发表日期: 1993年01期
• 关键词: 枯否细胞 前列腺素 E_2 肝切除 高精氨酸 肝再生 肝细胞 铜筛 实验观察 实质细胞

实验观察了肝切除后前列腺素E_2(PGE_2)与枯否细胞(KC)生成白介素6的关系。取S-D雄鼠分成70％肝切除和探查对照两大组,每组各再分成高精氨酸和低精氨酸RPMI-1640组织培养基(以下分别简称HA和LA培养基)两小组。分别在术后2、5、7和14天杀死动物,取出肝脏,用Hank平衡盐液(HBSS)灌洗,在铜筛中研碎至HBSS中,经沉淀、孵育等步骤取得肝细胞和非实质细胞,用HA或LA孵育肝细胞4小时,提取KC,其纯度>95％,活力>90％。

The relationship between prostaglandin E_2 (PGE_2) and interleukin-6 production by KCs was observed after hepatectomy. The male S-D rats were divided into two groups: 70% hepatectomy group and probing control group. Each group was subdivided into two groups: RPMI-1640 medium with high arginine concentration and low arginine concentration (hereinafter referred to as HA and LA medium respectively). The animals were sacrificed at days 2, 5, 7 and 14 after operation, the livers were removed, lavaged with Hanks Balanced Salt Solution (HBSS), pulverized into HBSS on a copper sieve, and the cells were harvested by precipitation and incubation Parenchymal cells were incubated with HA or LA for 4 hours. KC was extracted with> 95% purity and> 90% viability.