论文部分内容阅读
为建立更精确的一氧化氮（ Ｎ Ｏ）间接测定法，作者比较了镉屑、５ｍ ｍ ｏｌ／ Ｌ及 ８０ｍ ｍ ｏｌ／ Ｌ铜离子活化镉屑对镉还原法测定 Ｎ Ｏ 稳定代谢产物 Ｎ Ｏ２ － ／ Ｎ Ｏ３－ 的影响。结果显示：镉屑对 Ｎ Ｏ３－ 的还原效率明显优于铜离子活化镉屑（ Ｐ＜ ０．００１ ），还原率可达９６％ ，且在去蛋白细胞培养液中同样显示出较强的还原效率（ Ｐ＜ ０．０１）。结果提示：镉屑在生物液体中具良好的抗干扰性能。本法简便易行，不需昂贵设备，准确度较高，能较好的反映 Ｎ Ｏ２－ ／ Ｎ Ｏ３－ 的含量，适合一般实验室用于各种生物医学实验中的 Ｎ Ｏ 间接测定。
In order to establish a more accurate indirect measurement of nitric oxide (N O), the authors compared cadmium oxide, 5 m mol / L and 80 mol m L / - / N O3- influence. The results showed that the reduction efficiency of N 3+ by cadmium dross was significantly better than that of copper ion-activated cadmium dregs (P <0.001), the reduction rate was up to 96%, and also showed a strong reduction in the deproteinized cell culture medium Efficiency (P <0.01). The results suggest that: cadmium debris in biological fluids with good anti-interference performance. This method is simple and easy, does not require expensive equipment, high accuracy, can better reflect the content of N O2- / N O3-, suitable for general laboratory for a variety of biomedical experiments N O indirect determination.