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以改进方法从海带Laminaria japonica中提取褐藻淀粉,并人工磺化制得褐藻淀粉硫酸酯,得率分别为1%与2%,多糖含量为60.4%与31.1%,硫锰酯基含量为0.5%与10.2%,分子量为40000和80000。小鼠腹腔注射LD_(50)分别为980.00±110.215 mg/kg和689.80±80.93mg/kg,两种多糖均能明显促进小鼠腹腔巨噬细胞的吞噬功能,增强体液免疫功能,促进淋巴细胞转化,能对抗由环磷酰胺引起的白细胞下降和降低血清胆固醇等作用。褐藻淀粉还有对抗~(60)Coγ射线辐射,并对大鼠红细胞有明显促凝集作用,而褐藻淀粉硫酸酯对此作用不明显;但后者具有明显抗凝血,促进优球蛋白溶解等作用,而褐藻淀粉的作用不明显。
The brown algae starch was extracted from Laminaria japonica by the improved method, and the brown algae starch sulfate was obtained by artificial sulfonation. The yields were 1% and 2%, the contents of polysaccharides were 60.4% and 31.1%, and the content of thiocarbonate was 0.5% With 10.2% and molecular weight of 40,000 and 80,000. The intraperitoneal injection of LD_ (50) in mice was 980.00 ± 110.215 mg / kg and 689.80 ± 80.93 mg / kg, respectively. Both polysaccharides significantly promoted the phagocytosis of murine peritoneal macrophages, enhanced humoral immune function and promoted lymphocyte transformation , Can fight the cyclophosphamide caused by leukopenia and lower serum cholesterol and so on. Staphylococcus aureus was also resistant to ~ (60) Coγ-ray radiation and had a significant pro-agglutination on rat erythrocytes, whereas the brown algae starch sulfate had no obvious effect on this; however, the latter had obvious anti-coagulation and euglobulin solubilization Role, while the role of brown algae starch is not obvious.