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为进一步研究唐菖蒲杂交育种亲本选配和杂种的早期鉴定,以21个唐菖蒲品种为试材,对这些品种的表型性状特征进行聚类分析,并利用SRAP技术对其构建指纹图谱。结果表明:21个品种的表型性状表现出一定的遗传多样性,变异6.78%~147.29%。经过对SRAP-PCR反应条件的优化,从100对引物中筛选出16对引物适用于唐菖蒲PCR反应;通过对21个品种SRAP标记的试验与分析,共在468个位点上扩增出条带,多态性位点411个,平均每个引物组合扩增多态性条带25.7个;基于SRAP标记的UPGMA聚类分析显示,21个品种间Jaccardp’s相似系数0.46~0.75。将2种聚类结果进行比较发现,唐菖蒲品种的分类与表型性状无显著相关性,说明其品种具有复杂的遗传背景。
In order to further investigate the parental preference and early identification of hybrids in gladiolus, 21 varieties of gladiolus were used as materials to analyze the phenotypic traits of these varieties. The SRAP technique was used to construct the fingerprints of hybrids. The results showed that the phenotypic traits of 21 cultivars showed some genetic diversity with variation of 6.78% ~ 147.29%. After optimization of SRAP-PCR reaction conditions, 16 pairs of primers were screened from 100 pairs of primers for PCR reaction of gladiolus. Based on the SRAP markers of 21 cultivars, a total of 468 loci were amplified There were 411 polymorphic bands with an average of 25.7 polymorphic bands per primer combination. UPGMA cluster analysis based on SRAP markers showed that Jaccardp’s similarity coefficient was 0.46 ~ 0.75 among 21 cultivars. Comparing the two clustering results, we found that there is no significant correlation between the classification of gladiolus varieties and the phenotypic traits, indicating that the varieties have a complex genetic background.