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采用ＭＴＴ比色分析法和流式细胞仪检测不同浓度的雌三醇，不同浓度的他莫昔芬以及雌三醇加他莫昔芬对存在雌激素受体的ＨＴ－２９人结肠癌细胞株增殖的影响，结果显示雌三醇能提高细胞增殖率，其浓度对数值与此增殖率呈显著正相关（ｒ＝０．９７５，Ｐ＜０．００５），细胞周期各时相分布显示Ｓ期细胞数目明显增加；他莫昔芬有抑制细胞增殖作用，其浓度值与此增殖率呈显著负相关（ｒ＝－０．９５７，Ｐ＜０．０５）；他莫昔芬对雌三醇的增殖作用有部分抑制。结果提示雌三醇对雌激素受体阳性的ＨＴ－２９细胞株有增殖效应，这种效应能被雌激素受体拮抗剂他莫昔芬部分阻断，为临床用他莫昔芬治疗雌激素受体阳性的结肠癌提供了依据。
Different concentrations of estriol, tamoxifen at different concentrations, and estriol plus tamoxifen were tested for HT-29 human colon cancer cells in the presence of estrogen receptor using MTT colorimetric assay and flow cytometry. The effect of proliferation showed that estriol could increase the cell proliferation rate, and the logarithm of its concentration was significantly positively correlated with this proliferation rate (r=0.975, P<0.005), and the cell cycle showed phase S distribution at each phase. The number of cells increased significantly; tamoxifen inhibited cell proliferation, and its concentration value was significantly negatively correlated with this proliferation rate (r=-0.957, P<0.05); tamoxifen was also associated with estriol Proliferation is partially inhibited. The results suggest that estriol has a proliferative effect on the estrogen receptor-positive HT-29 cell line, and this effect can be partially blocked by the estrogen receptor antagonist tamoxifen, which is clinically used to treat estrogen with tamoxifen Receptor-positive colon cancer provides the basis.