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采用 DE-52 阴离子交换层析, 凝胶过滤高效液相层析(HPLC),Q—2阴离子交换快速液相层析(FPLC)技术从人胃粘膜中纯化了胃蛋白酶原Ⅰ,Ⅱ,建立了胃蛋白酶原Ⅰ,Ⅱ放射免疫分析方法。采用该方法测定了 190 例正常人及各种胃病患者的血清样品 PGⅠ,PGⅡ含量,并进行统计学分析, 与正常对照组相比,胃溃疡患者 PGⅠ略有升高而 PGⅡ明显升高(P<0.01), 十二指肠溃疡患者 PGⅠ明显升高(P<0.01),慢性萎缩性胃炎患者 PGⅠ水平有所下降(P<0.05),胃癌患者 PGⅠ,PGⅠ/PGⅡ明显下降(P<0.01),胃癌术后复发患者与胃癌术后患者相比 PGⅠ,PGⅡ水平都明显升高(P<0.01)。结果显示,血清胃蛋白酶原Ⅰ,Ⅱ的含量变化对胃癌的早期诊断及胃癌术后的随访具有较高的临床意义。
The pepsinogen I and II were purified from human gastric mucosa by DE-52 anion exchange chromatography, gel filtration high performance liquid chromatography (HPLC) and Q-2 anion exchange rapid liquid chromatography (FPLC). Pepsinogen I, II radioimmunoassay methods. Using this method, the serum samples PGI and PGII in 190 normal persons and various stomach patients were measured and statistically analyzed. Compared with the normal control group, the PGI in patients with gastric ulcer increased slightly and the PGII increased significantly (P <0.01), PGI in patients with duodenal ulcer was significantly increased (P <0.01), PGI levels in patients with chronic atrophic gastritis decreased (P <0.05), PGI, PGI/PGII in gastric cancer patients decreased significantly (P <0.01). The levels of PGI and PGII in patients with postoperative gastric cancer recurrence were significantly higher than those in postoperative patients with gastric cancer (P < 0.01). The results showed that the changes of serum pepsinogen I and II levels have a higher clinical significance for the early diagnosis of gastric cancer and follow-up of gastric cancer.