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目的:研究独一味和高乌甲素抗炎镇痛作用最佳配比。方法:采用小鼠醋酸扭体反应、小鼠水浴甩尾反应和小鼠巴豆油性耳肿试验,对独一味和高乌甲素进行正交优化试验,用金氏概率相加法研究不同剂量、不同比例的独一味和高乌甲素互相作用关系。结果:与模型对照组相比,独一味和高乌甲素单用均可以减少小鼠醋酸扭体次数(P<0.05),延长小鼠水浴甩尾反应潜伏期(P<0.05)和抑制小鼠耳廓肿胀度(P<0.05),起效剂量分别为56.25 mg/kg和0.375mg/kg。独一味与高乌甲素合用组可显著减少小鼠醋酸扭体次数(P<0.05,P<0.01),延长小鼠水浴甩尾反应潜伏期(P<0.05,P<0.01),明显抑制小鼠耳廓肿胀度(P<0.05,P<0.01),其中独一味和高乌甲素的配比为56.25:0.375抗炎镇痛作用最好。结论:高乌甲素与独一味联合使用,可减少高乌甲素的剂量,而镇痛效果不降低,在抗炎试验中,独一味与高乌甲素合用,可增强单独用药效果,也有增效作用。
Objective: To study the best ratio of anti-inflammatory and analgesic effect of only flavor and lappaconitine. Methods: The mice were subjected to acetic acid writhing reaction, mouse water bath flick-tail reaction and mouse crotonal oil-induced ear swelling test. Orthodox optimization experiments were carried out on the monosodium glutamate and lappaconitine, and the effects of different doses, Different proportions of the unique flavor and lappaconite interact with each other. Results: Compared with the model control group, monosodium glutamate alone and lappaconite alone could reduce the number of writhing in mice (P <0.05), prolong the latency of the tail-flick reaction (P <0.05) and inhibit the mice Auricle swelling degree (P <0.05), the effective dose were 56.25 mg / kg and 0.375 mg / kg respectively. The results showed that the combination of monosodium glutamate and lappaconitine could significantly reduce the number of acetic acid writhing in mice (P <0.05, P <0.01), and prolong the latency of tail-flick reaction in water bath (P <0.05, P <0.01) Auricle swelling degree (P <0.05, P <0.01), in which the ratio of monosodium glutamate and lappaconitine 56.25: 0.375, the best anti-inflammatory and analgesic effects. Conclusions: The combination of lappaconitine and monosodium glutamate can reduce the dosage of lappaconitine and the analgesic effect does not decrease. In the anti-inflammatory test, the combination of lavender and lappaconitine alone can enhance the effect of single drug, Synergy.