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目的 建立神经干细胞与骨髓基质细胞的共培养系统,根据该系统的条件性培养液诱导多巴胺能神经元的分化。方法 来源于胎脑海马、纹状体、额叶、中脑的神经干细胞与骨髓基质细胞建立起各自的共培养系统,并根据数种条件性培养液诱导神经干细胞的分化,以免疫细胞化学检测神经元的总体分化率及多巴胺能神经元的诱导率。结果 骨髓基质细胞及CO-BMSC能显著提高不同来源的神经干细胞的神经元分化率,同时只有中脑神经干细胞能被有效地进行多巴胺能神经元的诱导。结论 共培养系统诱发了神经干细胞与骨髓基质细胞的自/旁分泌作用,该作用可根据神经干细胞的区域特异性有效的定向诱导中脑神经干细胞的分化。
Objective To establish a co-culture system of neural stem cells and bone marrow stromal cells, and to induce the differentiation of dopaminergic neurons according to the conditioned medium of the system. METHODS: Neural stem cells derived from the hippocampus, striatum, frontal lobe, and midbrain of the fetal brain were cultured with bone marrow stromal cells to establish their own co-culture system. Neural stem cells were induced to differentiate according to several conditioned media. Immunocytochemistry The overall rate of neuronal differentiation and the induction of dopaminergic neurons. RESULTS: Bone marrow stromal cells and CO-BMSCs significantly increased the rate of neuronal differentiation of neural stem cells from different sources. Only mesencephalic neural stem cells could be efficiently induced by dopaminergic neurons. Conclusions Co-culture systems induce a self-paracrine effect of neural stem cells and bone marrow stromal cells, which can induce the differentiation of neural stem cells in the midbrain according to the region-specific and efficient targeting of neural stem cells.