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目的:探讨胰岛素样生长因子(IGF)-Ⅱ及IGF-Ⅱ-mRNA在肝癌组织中的表达特点及游离IGF-Ⅱ的临床价值。方法:以免疫组化法分析肝癌癌灶、癌周和远癌组织中IGF-Ⅱ的表达与胞内定位。从肝组织中制备总RNA,经随机引物和逆转录酶合成IGF-Ⅱ-cDNA,巢式聚合酶链反应扩增及DNA测序证实,分析IGF-Ⅱ-mR-NA的表达,并分析血清游离IGF-II水平。结果:肝癌组织IGF-Ⅱ表达呈强阳性,明显高于癌旁和远癌肝组织,分布呈胞浆包涵体样、全胞浆和核内三种形态。癌灶总RNA水平显著低于癌周与远癌组织;其IGF-Ⅱ灵敏度为2ng/L,扩增基因片段为170bp,癌灶和癌周组织中IGF-Ⅱ基因检出率,分别为100%和53.2%,远癌组织未见扩增片段。肝癌患者血游离IGF-II水平明显高于肝硬化和慢性肝炎患者。结论:IGF-Ⅱ及IGF-Ⅱ-mRNA的过度表达与肝癌形成有关,并有助于肝癌早期诊断。
Objective: To investigate the expression of insulin-like growth factor (IGF-Ⅱ) and IGF-Ⅱ-mRNA in hepatocellular carcinoma (HCC) and the clinical value of IGF-Ⅱ. Methods: Immunohistochemistry was used to analyze the expression of IGF-Ⅱ and intracellular localization in hepatocellular carcinoma, pericarcinoma and distant tissues. Total RNA was prepared from liver tissue, IGF-Ⅱ-cDNA was synthesized by random primers and reverse transcriptase, nested polymerase chain reaction amplification and DNA sequencing confirmed that IGF-Ⅱ-mR-NA expression was analyzed and serum free IGF-II levels. Results: The expression of IGF-Ⅱ in HCC tissues was strongly positive, which was significantly higher than that in adjacent and distant liver tissues. The distribution of IGF-Ⅱ was cytoplasmic inclusion bodies, all cytoplasm and nucleus. The total RNA level in cancer tissue was significantly lower than that in pericancerous and distant cancerous tissues. The sensitivity of IGF-Ⅱ was 2ng / L, and the amplified fragment was 170bp. The detection rates of IGF-Ⅱgene in cancer and pericancerous tissues were 100 % And 53.2% respectively. There were no amplified fragments in far cancer tissues. Patients with liver cancer serum IGF-II levels were significantly higher than cirrhosis and chronic hepatitis patients. Conclusion: The overexpression of IGF-Ⅱ and IGF-Ⅱ-mRNA is related to the formation of hepatocellular carcinoma and contributes to the early diagnosis of hepatocellular carcinoma.