Analysis of Gene Expression Pattern of Lumbar Intervertebral Disc Degeneration in Human

• 作 者: HU Ming MA Yuan-zheng FENG Hui
• 机 构: The Orthopaedic Department, PLA 309 Hospital, Beijing 100091,China
• 出 处: 中国康复理论与实践
• 发表日期: 2006年5期
• 关键词: intervertebral disc degeneration DNA microarray gene expression pattern
• 基金项目: 国家自然科学基金

Objective To investigate the gene expression changes in normal and degeneration lumbar intervertebral disc in humans, providing information for clinical. Methods The PCR products of 4096 human genes were spotted onto a kind of chemical-material-coated-glass slides. The total RNAs were isolated from the tissues. Both the mRNAs from the degeneration and normal lumbar intervertebral disc in humans were reversely transcribed to the cDNAs, which used as the hybridization probes with the incorporations of fluorescent dUTP. The mixed probes were then hybridized to the cDNA microarray. After high-stringent washing, the cDNA microarray was scanned for the fluorescent signals and analyzed with computer image analysis. Results Among the 4096 targets,there were 706 genes whose expression levels differed between the degeneration and normal lumbar intervertebral disc in all cases,comprising 298 up-regulated and 358 down-regulated ones. Conclusion DNA microarray technology is an effective technique in screening for differently expressed genes between the degeneration and normal lumbar intervertebral disc. Cell apoptosis plays an important role in the process of lumbar intervertebral disc degeneration.