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作者比较了多价小基因 DNA疫苗与全长蛋白疫苗诱导的 CD8+T细胞应答及保护效力 ,并证实了位于翻译起始密码子侧翼的Kozak序列对小基因疫苗的重要性。 作者用淋巴细胞性脉络丛脑膜炎病毒( LCMV)感染 8～ 1 2周龄 CB/ 6小鼠后 7天 ,取小鼠脾细胞与转染了 p CMV- SV(具有Kozak-
The authors compared the CD8 + T cell response and protective efficacy induced by the multivalent minigen DNA vaccine with the full length protein vaccine and confirmed the importance of the Kozak sequence flanking the translation initiation codon for a minigene vaccine. Seven days after inoculation of 8 to 12-week-old CB / 6 mice with lymphocytic choriomeningitis virus (LCMV), the mouse spleen cells were infected with pCMV-SV (with Kozak-