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目的:研究食管鳞状细胞癌中肝癌衍生生长因子(HDGF)、血管内皮生长因子(VEGF)的表达及其与微血管形成的关系。方法:通过免疫组化SABC法检测和比较68例食管鳞癌、20例切缘正常组织中HDGF、VEGF的表达和CD34标记的微血管密度(MVD),分析HDGF和VEGF表达之间的关系及其与食管鳞癌患者临床病理因素和食管癌组织MVD值的关系。结果:食管鳞癌组织中HDGF(63.2%)和VEGF(72.1%)的阳性表达率均明显高于切缘正常粘膜组织(15.0%、20.0%)(P<0.05),食管鳞癌组织和切缘正常粘膜组织中的MVD值分别为35.48±5.75和13.50±2.1(P<0.05)。食管鳞癌组织HDGF的阳性表达率仅与其临床分期明显相关(P<0.05),而VEGF的阳性表达率与其淋巴结转移、临床分期均显著相关(P<0.05),二者在食管鳞癌组织中的表达呈显著正相关(P<0.05)。食管鳞癌组织中HDGF、VEGF阳性表达组MVD值均明显高于HDGF、VEGF阴性表达组(P<0.05)。结论:HDGF可能通过诱导VEGF的产生,从而促进血管生成,参与食管鳞癌的发生、发展及转移。
Objective: To investigate the expression of hepatocellular carcinoma-derived growth factor (HDGF) and vascular endothelial growth factor (VEGF) in esophageal squamous cell carcinoma and its relationship with angiogenesis. Methods: The expressions of HDGF and VEGF and CD34-labeled microvessel density (MVD) in 68 cases of esophageal squamous cell carcinoma and 20 cases of normal marginal tissue were detected by immunohistochemical SABC method. The relationship between HDGF and VEGF expression was analyzed Relationship with clinicopathological parameters and MVD in esophageal squamous cell carcinoma patients. Results: The positive rates of HDGF (63.2%) and VEGF (72.1%) in esophageal squamous cell carcinoma were significantly higher than those in normal mucosa (15.0%, 20.0%) (P <0.05) MVD in marginal normal mucosa were 35.48 ± 5.75 and 13.50 ± 2.1, respectively (P <0.05). The positive expression rate of HDGF in esophageal squamous cell carcinoma tissues was only significantly correlated with its clinical stage (P <0.05), but the positive expression rate of VEGF was significantly correlated with lymph node metastasis and clinical stage (P <0.05), both in esophageal squamous cell carcinoma There was a significant positive correlation (P <0.05). The MVD values of HDGF and VEGF-positive esophageal squamous cell carcinoma tissues were significantly higher than those of HDGF and VEGF-negative groups (P <0.05). Conclusion: HDGF may induce angiogenesis by inducing the production of VEGF and participate in the occurrence, development and metastasis of esophageal squamous cell carcinoma.