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Human ileocecal adenocarcinoma cells HCT-8 were treated with RGD-containing cellular adhesion peptides including RGD,RGD(NH2)2(i.e.,RGE-NH2),RGDS,and RGDS-NH2. MTT assay was prepared to examine their inhibiting effects on HCT-8 cells after treatment. The methods including Haematoxylin and Eosin(HE) staining,transmission electron microscopy(TEM),immunohistochemistry,flow cytometry,and Reverse Transcription-Polymerase Chain Reaction(RT-PCR) were used to observe the morphology of the apoptotic cells and analyze the mechanism of apoptosis. The experimental results indicate that RGD-containing cellular adhesion peptides can inhibit the growth and proliferation of tumor HCT-8 cells in a dose-dependent manner and induce the apoptosis of HCT-8 cells. At the same time,the high conservative property of RGD was confirmed again.
Human ileocecal adenocarcinoma cells HCT-8 were treated with RGD-containing cellular adhesion peptides including RGD, RGD (NH2) 2 (ie, RGE-NH2), RGDS, and RGDS-NH2. MTT assay was prepared to examine their inhibiting effects on HCT -8 cells after treatment. The methods including Haematoxylin and Eosin (HE) staining, transmission electron microscopy (TEM), immunohistochemistry, flow cytometry, and Reverse Transcription-Polymerase Chain Reaction (RT- PCR) were used to observe the morphology of the apoptotic cells and analyze the mechanism of apoptosis. The experimental results indicate that RGD-containing cellular adhesion peptides can inhibit the growth and proliferation of tumor HCT-8 cells in a dose-dependent manner and induce the apoptosis of HCT-8 cells. At the same time, the high conservative property of RGD was confirmed.