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以高羊茅不育株ms6111和可育株MF111为试验材料,对其小孢子母细胞减数分裂和雄配子体发育过程及其相关的生理生化特征进行研究。结果表明,ms6111的小孢子母细胞从减数分裂前期I至单核靠边期明显存在着落后染色体、染色体桥和断片、微核、单价染色体、不均等分离、分裂不同步、游离染色体、三分体、五分体、60°纺锤体、染色体缺失、染色体减少等异常现象,初步分析这些异常现象是导致ms6111花粉败育的重要细胞学原因。造孢细胞期至散粉期,ms6111花药中的游离脯氨酸、可溶性蛋白、可溶性糖含量、SOD、CAT活性显著低于同时期的MF111,但MDA含量、POD活性却始终高于MF111,随着小孢子发育,MDA含量和POD活性呈逐步上升趋势。体内糖类物质、抗氧化酶代谢紊乱、游离脯氨酸亏损及MDA积累可能是引起ms6111小孢子败育的生理学原因之一。
The high-fescue plants MS6111 and fertile plants MF111 were used to study the microspore mother cell meiosis and male gametophyte development and their physiological and biochemical characteristics. The results showed that there were obviously backward chromosomes, chromosome bridges and fragments, micronuclei, monovalent chromosomes, unequal separation, dissociation of chromosomes, free chromosomes, one-thirds of chromosomes in MS6111 microspore mother cells from pre-meiotic stage I to single- Body, pentaprism, spindles at 60 °, chromosomal deletion and chromosomal abnormalities. The preliminary analysis of these abnormalities is an important cytological reason leading to the pollination of ms6111. During the period of spore-forming to sporadic, the contents of free proline, soluble protein, soluble sugar, SOD and CAT in anther of ms6111 were significantly lower than that of MF111 in the same period, but MDA content and POD activity were always higher than MF111. Microspore development, MDA content and POD activity showed a gradual upward trend. One of the physiological reasons causing MS6111 microspore abortion may be the in vivo carbohydrate, the metabolic disorder of antioxidant enzymes, free proline depletion and MDA accumulation.