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目的探讨羊膜对氧氟沙星滴眼液兔角膜通透性的影响。方法先给108只新西兰大白兔进行样本编号,再按号码单纯随机抽样并分为6组,每组18只,分别为正常角膜组、正常角膜羊膜移植组、机械刮除角膜上皮组、机械刮除上皮联合羊膜移植组、角膜碱烧伤组、角膜碱烧伤联合羊膜移植组。0．3％氧氟沙星眼液滴眼,每15 min滴眼1次,共4次,最后1次滴眼后5、30 min,1、2、4、6 h,于每个时间点每组18只大白兔中任意处死3只兔抽取房水。高效液相法检测房水中氧氟沙星的浓度。结果刮除角膜上皮组与正常角膜上皮组比较,药物的前房浓度均明显增高(均P<0．05)。正常角膜羊膜移植组5、30 min、1 h房水中氧氟沙星浓度与正常角膜组无明显差异(均P>0．05),而2、4、6 h则明显高于正常角膜组(均P<0．05);机械刮除上皮联合羊膜移植组2、4、6 h房水中氧氟沙星浓度亦明显高于机械刮除角膜上皮组(均P<0．05),而两组间5、30 min、1 h房水中氧氟沙星浓度比较无明显差异(均P>0．05);角膜碱烧伤联合羊膜移植组6 h以内房水中氧氟沙星浓度均高于碱烧伤组(均P<0．05)。结论羊膜具有一定药物蓄积和缓释作用,增加了氧氟沙星在角膜表面的停留时间,使药物的通透性增高。
Objective To investigate the effect of amniotic membrane on corneal permeability of ofloxacin eye drops. Methods A total of 108 New Zealand white rabbits were sampled and randomly divided into 6 groups (n = 18 each) according to the numbers, which were normal cornea group, normal corneal amniotic membrane transplantation group, mechanical scraping corneal epithelial group, mechanical scraping In addition to the epithelium combined with amniotic membrane transplantation group, corneal alkali burn group, corneal alkali burn combined with amniotic membrane transplantation group. 0.3% of ofloxacin eye drops, eye drops every 15 min 1, a total of 4 times, the last eye drops after 5,30 min, 1,2,4,6 h, at each time point Rabbits in each group of 18 rabbits were randomly sacrificed three rabbits drawn aqueous humor. Determination of ofloxacin in aqueous humor by high performance liquid chromatography. Results Compared with normal corneal epithelium, the concentration of drug in anterior chamber decreased significantly (all P <0.05). In the normal corneal amniotic membrane transplantation group, the concentrations of ofloxacin in aqueous humor at 5,30 min and 1 h were not significantly different from those in the normal cornea group (all P> 0.05), but were significantly higher at 2, 4 and 6 h than those in the normal cornea group (All P <0.05). The concentration of ofloxacin in aqueous humor of 2,4,6 h after mechanical curettage combined with amniotic membrane transplantation was also significantly higher than that of mechanical scraping of corneal epithelium (both P <0.05) The concentrations of ofloxacin in aqueous humor at 5,30 min and 1 h were not significantly different (all P> 0.05). The concentration of ofloxacin in aqueous humor within 6 h after alkali burn and amniotic membrane transplantation was higher than that of base Burn group (all P <0.05). Conclusions Amniotic membrane has certain drug accumulation and sustained release effect, which increases the stay time of ofloxacin on the corneal surface and increases the permeability of the drug.