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考察了高压均质提取柴达木枸杞叶有效成分的最佳工艺及对有效成分进行了纯化。在乙醇体积分数、料液比〔枸杞叶质量与乙醇溶剂体积比值(g/mL),下同〕、均质压力及提取时间4个单因素实验的基础上利用正交设计对实验进行优化,得出高压均质提取法的最佳工艺条件为:乙醇体积分数80%,料液比1∶10,均质压力60MPa,提取时间30 min,在该条件下,提取物中芦丁质量分数为10.53%,总黄酮质量分数为32.61%。在该条件下分离纯化工艺为:选用AB-8大孔树脂,水洗脱用量5 BV,乙醇洗脱用量4 BV,经纯化后的产物中,芦丁质量分数可达60.85%,总黄酮质量分数可达90.53%。
The optimum conditions of high pressure homogenization extraction of the active ingredients of Lycium barbarum leaves were investigated and the active components were purified. The orthogonal design was used to optimize the experiment on the basis of four single factor experiments, including volume fraction of ethanol, mass ratio of wolfberry leaf to ethanol volume (g / mL), homogenization pressure and extraction time, The optimum conditions of high pressure homogenization extraction were obtained as follows: the volume fraction of ethanol was 80%, the ratio of material to liquid was 1:10, the pressure of homogenization was 60 MPa, the extraction time was 30 min. Under this condition, the mass fraction of rutin in the extract was 10.53%, the total flavonoids content was 32.61%. Under these conditions, the separation and purification process was: selection of AB-8 macroporous resin, the amount of water elution 5 BV, the amount of ethanol elution 4 BV, the purified product, the mass fraction of rutin up to 60.85%, the total flavonoid mass Score up to 90.53%.