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目的建立HPLC波长切换法测定牛黄清感胶囊中绿原酸、黄芩苷、连翘苷3个成分的含量。方法采用Agilent ZORBAX Eclipse XDB-C18(4.6mm×150mm,5μm)色谱柱,以乙腈(A)-0.1%磷酸溶液(B)为流动相,梯度洗脱(0~5min,5%A;5~15min,5%~18%A;15~25 min,18%~28%A);波长:0~10.0 min,327nm;10.0~16.5 min,280nm;16.5~25.0min,277nm;流速:1.0 ml·min-1;柱温:30℃;进样量:10μl。结果绿原酸、黄芩苷、连翘苷的线性范围分别是0.1063~2.126μg(r=0.9998)、0.3007~6.013μg(r=0.9999)、0.05180~1.036μg(r=0.9996);平均加样回收率(n=6)分别为98.01%、98.83%、98.04%,RSD分别为1.31%、0.77%、1.29%。结论该方法操作简单,重复性好,可用于牛黄清感胶囊的质量控制。
OBJECTIVE To establish HPLC wavelength-switching method for the determination of chlorogenic acid, baicalin and forsythin in Niuhuang Qingyan Capsule. Methods The mobile phase consisted of Agilent ZORBAX Eclipse XDB-C18 column (4.6 mm × 150 mm, 5 μm) and acetonitrile (A) -0.1% phosphoric acid solution (B) 15 min, 25 min, 18% -28% A); wavelength: 0-10.0 min, 327 nm; 10.0-16.5 min, 280 nm; 16.5-25.0 min, 277 nm; min-1; column temperature: 30 ℃; injection volume: 10μl. Results The linear ranges of chlorogenic acid, baicalin and forsythin were 0.1063 ~ 2.126μg (r = 0.9998), 0.3007 ~ 6.013μg (r = 0.9999) and 0.05180 ~ 1.036μg Rates (n = 6) were 98.01%, 98.83% and 98.04%, respectively, with RSDs of 1.31%, 0.77% and 1.29%, respectively. Conclusion The method is simple, reproducible and can be used for the quality control of Niuhuang Qinggan capsule.