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目的 探讨血小板源生长因子ｂｂ（ＰＤＧＦｂｂ） 、转化生长因子β（ＴＧＦβ） 对人腹膜间皮细胞（ＨＰＭＣ）增殖及合成细胞外基质（ＥＣＭ） 的影响。方法 建立ＨＰＭＣ体外培养体系。３ＨＴｄＲ 掺入法测定细胞增殖；放射免疫法检测细胞上清中Ⅲ型前胶原（ ⅢＰＣ） 。结果 ＰＤＧＦｂｂ 促进、但ＴＧＦβ抑制ＨＰＭＣ增殖，均呈剂量依赖性（ Ｐ＜ ０－０１） 。２ 者均能刺激ＨＰＭＣ 产生ⅢＰＣ（ Ｐ＜ ０－０１）。结论 ＣＡＰＤ相关性腹膜炎时腹腔中高浓度的ＰＤＧＦｂｂ 、ＴＧＦβ能调节间皮层的修复和重塑，刺激其合成分泌ＥＣＭ，后者可能是腹膜炎（ＰＩ）反复发生最终导致腹膜硬化的重要机制之一。
Objective To investigate the effect of platelet-derived growth factor-bb (PDGF-bb) and transforming growth factor-β (TGF-β) on the proliferation of human peritoneal mesothelial cells (HPMC) and the synthesis of extracellular matrix (ECM). Methods HPMC in vitro culture system was established. Cell proliferation was measured by 3H-TdR incorporation and type III procollagen (ⅢPC) was detected by radioimmunoassay. Results PDGF bb promote, but TGF β inhibition of HPMC proliferation, were dose-dependent (P <0-01). Both of them could stimulate HPMC to produce ⅢPC (P <0-01). Conclusion CAPD-related peritonitis in the peritoneal high concentration of PDGF bb, TGF β can regulate the repair and remodeling of the mesothelial cells to stimulate their synthesis and secretion of ECM, the latter may be repeated peritonitis (PI) and eventually lead to an important mechanism of peritoneal sclerosis one.