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目的和方法：观察猪肝部分切除后２４、４８和７２ｈ颈外静脉及７２ｈ肝静脉和门静脉的血清，以及术后７２ｈ从再生肝制得的肝细胞生长因子（ｐｉｇｒｅｇｅｎｅｒａｔｉｎｇｈｅｐａｔｏｃｙｔｅｇｒｏｗｔｈｆａｃｔｏｒ，ＰＲＨＧＦ）和它们的不同浓度对体外原代培养肝细胞生长的影响。结果：加入术后上述静脉血清均具有明显的促进体外培养肝细胞生长的作用。其中肝静脉血清的作用最强、门静脉血清的次之，颈外静脉血清的作用居第三，而且当加入浓度上升至８０％时，它们的促肝细胞生长作用与５０％浓度相比均无显著降低，相反，加入术前颈外静脉血清浓度达８０％时，其刺激肝细胞生长作用则明显降低。ＰＲＨＧＦ的促进肝细胞生长作用在一定范围内呈剂量依赖性增强。当加入浓度为０３４ｍｇ蛋白质／孔时，达到顶峰，此后即逐渐下降。加入浓度为０４９ｍｇ蛋白质／孔时，则出现抑制效应。结论：术前正常猪颈外静脉血清和ＰＲＨＧＦ除有刺激肝细胞生长作用外，尚有抑制肝细胞生长的效应，当所用浓度增加达到一定值时，即对原代培养肝细胞生长呈现抑制效应
PURPOSE AND METHODS: The serum levels of the external jugular vein and the hepatic vein and portal vein at 24 h, 48 h and 72 h after partial hepatectomy were observed, and the changes of pigregenerating hepatocyte growth factor (PRHGF) and their difference Effect of Concentration on Growth of Primary Cultured Hepatocytes in. Results: After the above-mentioned venous serum was added, it obviously had the effect of promoting the growth of hepatocytes in vitro. Among them, hepatic vein serum had the strongest effect, followed by portal vein serum and external jugular vein serum in the third place, and when the added concentration increased to 80%, their pro-hepatocyte growth effect had no effect compared with 50% concentration Significantly reduced, on the contrary, adding preoperative external jugular vein serum concentration of 80%, its role in stimulating hepatocyte growth was significantly reduced. PRHGF promote hepatocyte growth in a dose-dependent manner in a dose-dependent manner. When adding the concentration of 034mg protein / hole, peaked, and then gradually decreased. When added at a concentration of 0.49 mg protein / well, the inhibitory effect appears. Conclusions: Preoperative normal external jugular vein serum and PRHGF can not only stimulate the growth of hepatocytes, but also inhibit the growth of hepatocytes. When the concentration of PRHGF increases to a certain value, it can inhibit the growth of primary hepatocytes