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目的 :探讨变应性鼻炎 (AR)大鼠鼻黏膜凋亡相关基因Bcl 2mRNA及其蛋白的表达 ,以进一步了解变应性鼻炎的发病机制。方法 :将 2 0只大鼠随机分为实验组和对照组 ,每组 10只。实验组以卵清蛋白腹腔注射致敏 ,继之鼻局部激发建立变应性鼻炎动物模型。取实验组与对照组动物鼻呼吸区黏膜 ,行Bcl 2mRNA原位杂交染色、Bcl 2与Bax蛋白免疫组化染色 ,并行苏木精 伊红染色以资对比。结果 :实验组Bcl 2mRNA与Bcl 2蛋白主要表达于鼻黏膜腺体细胞 ,其次是上皮层 ,鼻分泌物中可见大量呈强阳性反应的嗜酸性粒细胞 ,Bcl 2mRNA与Bcl 2蛋白表达水平显著高于对照组 (P <0 .0 5 ) ,Bax蛋白与Bcl 2蛋白表达部位一致 ,两组表达水平差异无统计学意义 (P >0 .0 5 )。结论 :变应性鼻炎大鼠鼻黏膜凋亡调控基因Bcl 2mRNA及其蛋白表达上调 ,是变应性鼻炎鼻分泌物增多及鼻黏膜上皮破坏的机制之一
Objective: To investigate the expression of Bcl-2 mRNA and protein in the nasal mucosa of allergic rhinitis (AR) rats in order to further understand the pathogenesis of allergic rhinitis. Methods: Twenty rats were randomly divided into experimental group and control group, with 10 rats in each group. Experimental group with ovalbumin intraperitoneal injection sensitization, followed by local nasal stimulation to establish an animal model of allergic rhinitis. The mucosa of the nasal respiratory area of the experimental group and the control group were examined by Bcl-2 mRNA in situ hybridization staining, Bcl-2 and Bax protein immunohistochemical staining, and hematoxylin-eosin staining for comparison. Results: Bcl-2 mRNA and Bcl-2 protein were mainly expressed in the glandular cells of the nasal mucosa, followed by the epithelial layer. In the nasal secretions, a large number of eosinophils were found with strong positive reaction. The expressions of Bcl-2 mRNA and Bcl-2 protein were significantly higher In the control group (P <0.05), the expression of Bax protein and Bcl 2 protein were consistent, and there was no significant difference between the two groups (P> 0.05). CONCLUSIONS: Bcl-2 mRNA and protein expression in nasal mucosa of allergic rhinitis rats is upregulated, which is one of the mechanisms of allergic rhinitis nasal secretions and nasal mucosa epithelial destruction