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目的对肉鸡养殖和屠宰环节进行沙门菌监测,确定肉鸡在养殖和屠宰环节中沙门菌的污染状况,分析肉鸡中沙门菌的污染来源,为食品安全风险评估提供基础数据,同时也为预防和控制由沙门菌起的食源性疾病提供科学依据。方法在四川省成都市、遂宁市选取肉鸡养殖场和屠宰场作为监测点。采集肉鸡活体肛拭、养殖场环境样本,肉鸡胴体,屠宰场环境样本进行沙门菌分离,并对检出菌株运用脉冲场凝胶电泳(PFGE)进行分子分型,用微量肉汤稀释法进行14种抗生素药物敏感实验。结果共从2个肉鸡屠宰场和1个肉鸡养殖场采集样本417份,检出沙门菌12株,检出率为2.87%。通过PFGE分型,共得到4个带型。其中遂宁养殖场的1株环境分离株和遂宁屠宰场的3株肉鸡胴体分离株具有相同PFGE型别,成都屠宰场的2株环境分离株和该屠宰场4株肉鸡胴体分离株具有相似PFGE型别。遂宁分离的4株沙门菌对14种抗生素100%敏感。成都分离的8株沙门菌对14抗生素耐药程度各异,其中4株沙门菌能耐受3种以上药物。结论四川省部分地区肉鸡在养殖和屠宰环节存在沙门菌污染,且有多重耐药菌株出现,应进一步加大监测力度。
Objective To monitor Salmonella in broiler breeding and slaughter, determine the contamination status of Salmonella in breeding and slaughtering sectors, analyze the sources of Salmonella contamination in broilers and provide the basic data for food safety risk assessment, and also to prevent and control Food-borne diseases caused by Salmonella provide a scientific basis. Methods Chickens farms and slaughterhouses were selected as monitoring points in Chengdu and Suining, Sichuan Province. Salmonella swabs were collected from live broilers, environmental samples of farms, carcass of broilers and slaughterhouse samples were isolated. Salmonella isolates were isolated by pulsed-field gel electrophoresis (PFGE) and sequenced by micro broth dilution method Antibiotic drug sensitive experiment. Results A total of 417 samples were collected from 2 broiler slaughterhouses and 1 broiler farm. 12 strains of Salmonella were detected with a detection rate of 2.87%. By PFGE typing, a total of four bands. One isolates from Suining farm and three broiler carcasses from Suining slaughterhouse had the same PFGE type, two isolates from Chengdu slaughterhouse and four broiler carcasses from slaughterhouse had similar PFGE patterns do not. Four strains of Salmonella isolated from Suining were 100% sensitive to 14 antibiotics. The 8 strains of Salmonella isolated in Chengdu had different degrees of resistance to 14 antibiotics, of which 4 Salmonella strains were able to tolerate 3 or more drugs. Conclusion There are Salmonella contamination in broiler breeding and slaughter areas in some areas of Sichuan Province and multiple drug-resistant strains appear. Monitoring efforts should be further intensified.