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目的:观察穿心莲内酯对异丙肾上腺素所致大鼠心肌肥厚的保护作用及其对心肌组织Na+-K+-ATPase、Ca2+-Mg2+-ATPase活性及羟脯氨酸含量的影响。方法:采用ISO1 mg.kg-1.d-1,背部皮下注射,连续10 d,建立大鼠心肌肥厚模型。造模第2天开始给予不同浓度的穿心莲内酯、二甲基亚砜或NS,连续14 d,末次给药后禁食12 h,称体质量,麻醉,取心脏,称全心及左心室质量,计算左心室质量指数,测定心肌组织Na+-K+-ATPase、Ca2+-Mg2+-ATPase活性及羟脯氨酸含量。结果:模型组左心室质量指数升高(P<0.01),心肌组织Na+-K+-ATPase(P<0.01)、Ca2+-Mg2+-ATPase(P<0.01)活性降低,羟脯氨酸含量升高(P<0.01);与模型组相比,低剂量组左心室质量指数降低(P<0.01),高剂量及低剂量穿心莲内酯治疗组心肌组织Na+-K+-ATPase、Ca2+-Mg2+-ATPase活性升高,羟脯氨酸含量均降低,且呈现剂量依赖性效应。结论:穿心莲内酯可通过提高Na+-K+-ATPase、Ca2+-Mg2+-ATPase的活性,降低羟脯氨酸含量,从而抑制心脏纤维化,起抗心肌肥厚作用。
OBJECTIVE: To observe the protective effect of andrographolide on isoproterenol-induced cardiac hypertrophy in rats and its effect on the activity of Na + -K + -ATPase, Ca2 + -Mg2 + -ATPase and hydroxyproline in myocardium. Methods: The rat model of cardiac hypertrophy was established by subcutaneous injection of ISO1 mg.kg-1.d-1 for 10 days. Starting from day 2, different concentrations of andrographolide, dimethyl sulfoxide and NS were given for 14 consecutive days. After the last administration, they were fasted for 12 hours. Body mass, anesthesia, heart, heart and left ventricle The left ventricular mass index was calculated and the activities of Na + -K + -ATPase, Ca2 + -Mg2 + -ATPase and hydroxyproline in myocardium were measured. Results: The left ventricular mass index increased (P <0.01), the activity of Na + -K + -ATPase (P <0.01) and Ca2 + -Mg2 + -ATPase (P <0.01) decreased and the hydroxyproline content increased (P <0.01). Compared with model group, the left ventricular mass index decreased in low dose group (P <0.01). The activities of Na + -K + -ATPase, Ca2 + -Mg2 + -ATPase in high and low dose of andrographolide group were significantly increased High, hydroxyproline content decreased, and showed a dose-dependent effect. Conclusion: Andrographolide can inhibit cardiac fibrosis and resist cardiac hypertrophy by increasing the activity of Na + -K + -ATPase, Ca2 + -Mg2 + -ATPase and decreasing the hydroxyproline content.