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目的:考察粒径对微胶囊强度及微囊化细胞生长代谢的影响,为制备性能优良的生物微胶囊提供实验依据。方法:制备不同粒径的凝胶珠,测定其在相同成膜条件下的球磨强度,进而用台盘蓝拒染法测定微囊化细胞的增殖及活率。结果:小粒径的微胶囊具有更厚的微囊膜及更高的球磨强度,另外小粒径微胶囊培养细胞能够获得更多的细胞数(350μm,570μm和900μm微囊内的细胞数量分别为:5.67×107、4.71×107和3.89×107/mL microcapsule,P<0.05)及更高的细胞活率(350μm、570μm和900μm微胶囊的细胞活率分别为:83.70%、67.64%和75.73%,P<0.05)。结论:粒径能影响微胶囊的强度及微囊化细胞的生长、代谢。
OBJECTIVE: To investigate the effect of particle size on the microcapsule strength and the growth and metabolism of microencapsulated cells, and to provide experimental evidence for the preparation of bio-microcapsules with good performance. Methods: Gel beads with different particle sizes were prepared and their milling strength was measured under the same film-forming conditions. Then the proliferation and viability of the microencapsulated cells were determined by the plate blue staining method. Results: The microcapsules with smaller particle size had thicker microcapsules and higher ball milling strength. In addition, the number of cells in microcapsules cultured in small particle size (350μm, 570μm and 900μm respectively) : 5.67 × 107,4.71 × 107 and 3.89 × 107 / mL microcapsule, P <0.05) and higher cell viability (cell viability of 350μm, 570μm and 900μm microcapsules were 83.70%, 67.64% and 75.73 %, P <0.05). Conclusion: The particle size can affect the strength of microcapsules and the growth and metabolism of microencapsulated cells.