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应用医用吸附剂直接吸附血浆中的TNFα是一种高效的分离方法。选用 8种不同结构和性质的氨基酸修饰大孔吸附树脂NK 110 ,通过对TNFα吸附量的测定 ,吸附动力学曲线和吸附等温线的描述等方法 ,研究了经修饰后大孔吸附树脂对血浆中TNFα的吸附性能 ,结果表明 :1.半胱氨酸修饰的NK 110对TNFα的吸附量高 ,静态吸附12 0min时达 76 83 80u mL。 2 .其吸附动力学曲线表明 ,经半胱氨酸修饰后 ,相同时间内吸附量更高 ,且二者吸附量有差别 (P <0 0 1)。 3.由吸附等温线可见NK 110对血浆中TNFα的吸附量随着溶液浓度的升高而升高 ,但其吸附率随之呈降低趋势。经半胱氨酸修饰后 ,其吸附量随着TNFα浓度的升高而升高 ,吸附率基本不变。
The direct adsorption of TNFα in plasma using medical adsorbent is an efficient separation method. Eight kinds of amino acid modified macroporous adsorption resin NK 110 with different structures and properties were selected and the modified macroporous adsorption resin was studied in plasma by measuring the adsorption amount of TNFα, adsorption kinetic curve and adsorption isotherm. The adsorption performance of TNFα showed that: 1. The cysteine-modified NK 110 has a high adsorption capacity for TNFα, and the static adsorption reaches 76 83 80 μ mL at 120 min. 2. The adsorption kinetics curve showed that after cysteine modification, the adsorption amount was higher in the same time, and the adsorption amount of the two was different (P<0 01). From the adsorption isotherm, the adsorption amount of TNFα in plasma of NK 110 increased with the increase of solution concentration, but the adsorption rate of NK 110 decreased. After cysteine modification, the adsorption amount increased with the increase of TNFα concentration, and the adsorption rate remained basically unchanged.