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本文介绍作者在卫生检验中对细菌集落计数的一些经验,供同志们参考。一、检品稀释与菌落数: 在细菌集落计数对,适宜的检样稀释度是重要的,因为平板上菌落数过多时,菌落相互重叠及拮抗的机会增加,菌落数过少又具有偶然性,两者都影响检验结果的正确性。一个平板上的菌落数一般以在30～300之间较宜。在记录检品菌落数时,应注明稀释倍数,以利判断。例如有二个检样,一个平板上有500个菌落,
This article describes some of the authors’ experience in counting bacterial colonies in health exams for your reference. First, the seizure of dilution and the number of colonies: colonies in the bacterial count, the appropriate dilution of the sample is important, because the number of colonies on the plate too much, the colony overlap and the chance of increased antagonism, the number of colony is too small and accidental, Both affect the correctness of the test results. The number of colonies on a plate is generally between 30 and 300. In recording the number of test colony, should be noted that the dilution multiple, to facilitate judgment. For example, there are two samples, 500 colonies on a plate,