论文部分内容阅读
Objective:To explore the effects of Livin gene knock down using sequence-specific siRNA on apoptosis of human breast cancer cell ZR-75-30.Methods:Chemically synthesized double stranded RNA(dsRNA)targeting Livin was transfected into human breast cancer cell ZR-75-30 with lipofectamineTM2000.The transfection efficiency was observed under a fluorescence confocal microscope.Expression of Livin at both mRNA and protein levels were detected by reverse transcription-polymerase chain reaction(RT-PCR)and immunohistochemical analysis.The effects on apoptosis of ZR-75-30 cells were assessed by FCAS.Results:The Livin siRNA can effectively and specifically inhibited the expression of Livin gene in ZR-75-30.The inhibition rate was 53.66%at mRNA level and 58.32%at protein level.After 24h,(8.36±0.20)%cells transfected with siRNA were induced to apoptosis.Conclusion:Chemically synthesized short Livin-siRNA Call effectively inhibit Livin over expression and remarkably induce apoptosis in human breast cancer cell line ZR-75-30.Livin RNAi has a potential value in gene therapy of breast cancer.